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http://phatraff.com/posts/hypertension/diovan.php - order cheap diovan pills 6. Carefully remove the supernatent, place the tube onto a vortexer set at a low “shake” speed and add cold fixative one drop at a time (see Note 7). The pellet should darken immediately. After addition of 20 or so drops the pellet is fixed, and fixative can be added in larger volumes. Make the final volume up to 10 mL. Occasionally, you may be asked to draw an epidemic curve when you don’t know either the disease or its incubation time. In that situation, it may be useful to draw several epidemic curves with different units on the x-axis to find one that best portrays the data. For example, the epidemic curves shown in Figures 6.2b and 6.2c display the same data as in Figure 6.2a; the x-axis is measured in units of 12 hours in Figure 6.2a, 6 hours in Figure 6.2b, and 24 hours (1 day) in 6.2c. Figure 6.2d shows the same data one more time, but with stacks of squares that each represent one case.
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